Prevalence and Genetic Characterization of Carbapenem- and Polymyxin-Resistant Acinetobacter baumannii Isolated from a Tertiary Hospital in Terengganu, Malaysia

Zarizal, Suhaili and Salwani, Ismail and Chew Chieng, Yeo and Nor Iza, A.rahman (2014) Prevalence and Genetic Characterization of Carbapenem- and Polymyxin-Resistant Acinetobacter baumannii Isolated from a Tertiary Hospital in Terengganu, Malaysia. ISRN Microbiology. pp. 1-9. ISSN 2356-7872

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Abstract

Nosocomial infection caused by Acinetobacter baumannii is of great concern due to its increasing resistance tomost antimicrobials. In this study, 54 nonrepeat isolates of A. baumannii from the main tertiary hospital in Terengganu, Malaysia, were analyzed for their antibiograms and genotypes. Out of the 54 isolates, 39 (72.2%) were multidrug resistant (MDR) and resistant to carbapenems whereas 14 (25.9%) were categorized as extensive drug resistant (XDR) with additional resistance to polymyxin B, the drug of “last resort.” Pulsed-field gel electrophoresis analyses showed that the polymyxin-resistant isolates were genetically diverse while the carbapenem-resistant isolates were clonally related. The 14 XDR isolates were further investigated for mutations in genes known to mediate polymyxin resistance, namely, pmrCAB, and the lipopolysaccharide biosynthesis genes, lpxA, lpxC, lpxD, and lpsB. All 14 isolates had a P102H mutation in pmrA with no mutation detected in pmrC and pmrB. No mutation was detected in lpxA but each polymyxin-resistant isolate had 2–4 amino acid substitutions in lpxD and 1-2 substitutions in lpxC. Eight resistant isolates also displayed a unique H181Y mutation in lpsB.The extent of polymyxin resistance is of concern and the novel mutations discovered here warrant further investigations.

Item Type: Article
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions: Faculty of Bio-resources & Food Industry
Depositing User: Rafidah Saaid
Date Deposited: 02 Jan 2022 04:12
Last Modified: 02 Jan 2022 04:18
URI: http://eprints.unisza.edu.my/id/eprint/4257

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