Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines

Manaf, Ali (2012) Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines. Asian Pacific journal of cancer prevention. pp. 5131-5136. ISSN 15137368

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Abstract

Cancer is one of the major health problems worldwide and its current treatments have a number of undesired adverse side effects. Natural compounds may reduce these. Currently, a few plant products are being used to treat cancer. In this study, goniothalamin, a natural occurring styryl-lactone extracted from Goniothalamus macrophyllus, was investigated for cytotoxic properties against cervical cancer (HeLa), breast carcinoma (MCF-7) and colon cancer (HT29) cells as well as normal mouse fibroblast (3T3) using MTT assay. Fluorescence microscopy showed that GTN is able to induce apoptosis in HeLa cells in a time dependent manner. Flow cytometry further revealed HeLa cells treated with GTN to be arrested in the S phase. Phosphatidyl serine properties present during apoptosis enable early detection of the apoptosis in the cells. Using annexin V/PI double staining it could be shown that GTN induces early apoptosis on HeLa cells after 24, 48 and 72 h. It could be concluded that goniothalamin showing a promising cytotoxicity effect against several cancer cell lines including cervical cancer cells (HeLa) with apoptosis as the mode of cell death induced on HeLa cells by Goniothalamin was

Item Type: Article
Uncontrolled Keywords: Goniothalamin - HeLa cervical cancer cell line - fluorescence microscopy - cellular DNA content - apoptosis
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Bio-resources & Food Industry
Depositing User: Mrs Norhidayah Razak
Date Deposited: 12 Jan 2021 03:18
Last Modified: 21 Aug 2021 08:25
URI: http://eprints.unisza.edu.my/id/eprint/2448

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