Scheme of obtaining β-carotene standard from pumpkin (Cucurbita moschata) flesh

Norshazila, Shahidan (2012) Scheme of obtaining β-carotene standard from pumpkin (Cucurbita moschata) flesh. International food research journal. pp. 531-535. ISSN 19854668

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Abstract

Characterization and quantification of carotenoid compound is complicated. costly and timeconsuming. The accuracy and reliability of the data depend solely on the standard and to High Performance Liquid Chromatography (HPLC) analysis but the major constraint is to acquire and to maintain the pure standards Carotenoid standards are commercially available but they are expensive and are prone to isomerization and oxidation. Thus. the purpose of this study is to establish an analytical method for isolating a-carotene by using open column chromatography (OCC) from pumpkin (Cucurbita moschata) to be used as one of the carotenoid standards for determination of total and individual carotenoid Pumpkin with orange flesh has been chosen due to the non-seasonal nature and its availability all year-round This study demonstrated that the purity of a-carotene standard-determined by HPLC was ranged from 92 21 to 97 95% The standard curves with five different concentrations of 13-carotene extract from pumpkins in triplicate were constructed by plotting the peak area against the concentration The coefficient of correlation was 0 9936 Therefore this study established that pumpkin can be a reliable source of beta-carotene standard as it is cheap and commonly available throughout the year

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