Md. Sarwar, Jahan and Okuma, E1 and Munemasa, S and Hossain, MA Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis. Journal of plant physiology. ISSN 2048-2055
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Abstract
We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1- chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomatal closure. Decreasing GSH by both the cad2-1 mutation and CDNB treatment enhanced ABAinduced stomatal closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca 2+ -permeable channel currents by ABA or oscillation of the cytosolic free Ca 2+ concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca 2+ oscillation in ABA signal pathway of Arabidopsis guard cells.
Item Type: | Article |
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Uncontrolled Keywords: | Abscisic acid; Cytosolic calcium; Glutathione; Reactive oxygen species; Stomatal closure |
Subjects: | S Agriculture > SB Plant culture |
Divisions: | Faculty of Bio-resources & Food Industry |
Depositing User: | Mrs Norhidayah Razak |
Date Deposited: | 14 Dec 2020 03:46 |
Last Modified: | 15 Aug 2021 03:02 |
URI: | http://eprints.unisza.edu.my/id/eprint/2141 |
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