Assessment of TRPV4 ion channel and its role in colorectal cancer cells

Siti Yusrina Nadihah, Jamaludin and Mohd Hilmi, Abu Bakar and Mohd Nizam, Zahary and Nor Hidayah, Abu Bakar and Abd Manaf, Ali and Nurul Nadiah, Bahari and Aisyah Hasyila, Jahidin (2018) Assessment of TRPV4 ion channel and its role in colorectal cancer cells. In: 3rd International Conference on Molecular Diagnostics & Biomarker Discovery 2018, 07 Oct 2018, Penang, Malaysia.

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Abstract

The transient receptor potential (TRP) channels are non-selective cation channels that consist of seven subgroups displaying different ion selectivity, physiological functions and activation mechanisms. TRP vanilloid 4 (TRPV4) is a member of TRPV family which shows permeability to both Ca2+ and Na+. It is activated by diverse stimuli including both physical and chemical. TRPV4 is found in various types of tissues such as kidneys, airway smooth muscle and lungs, where it participates in many cellular processes including mechanosensation, cell volume regulation and osmoregulation. This study aimed at investigating the potential role of TRPV4 in colorectal cancer cells. Real time RT-PCR was performed to evaluate TRPV4 mRNA levels in HT-29 and HCT-116 human colorectal cancer cells in comparison with the normal colon CCD-18Co cells. The MTT colorimetric assay was done to assess the effect of a TRPV4 activator GSK1016790A and a TRPV4 inhibitor RN 1734 on the proliferation of human colorectal cancer cells. Real-time RT-PCR analysis revealed that TRPV4 mRNA levels were higher in the normal colon CCD-18Co cells than HT-29 cells. TRPV4 mRNA was undetected in HCT-116 cells. TRPV4 activation by GSK1016790A increased the viability of HT-29 cells while inhibition of TRPV4 using RN 1734 reduced the viability of this cell line. These data suggest that TRPV4 is differentially expressed in human colorectal cancer cells and that pharmacological modulation of TRPV4 exerts differential effects on the proliferation of HT-29 cells. Further functional studies are required to define the possible mechanisms involved.

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