Detection, Isolation and Antibiotic Testing of Vibrio cholerae in seafood

John Tang, Yew Huat and Nurul Suhada, Sudin (2018) Detection, Isolation and Antibiotic Testing of Vibrio cholerae in seafood. Journal of Agrobiotechnology, 9 (1). pp. 50-57. ISSN 2180-1983

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Vibrio cholerae is important water borne human pathogen that associated with cholera disease. The goal of this study was to determine the prevalence and antimicrobial resistance of V. cholerae found in seafood. Samples of prawn, cockles and squid samples bought from Kuala Terengganu and Besut markets. The detection of V. cholerae in the samples was done using Polymerase Chain Reaction (PCR) assay and plating method. V. cholerae isolates were subjected to six antibiotics susceptibility test using Kirby Bauer method. In Besut district, V. cholerae was found in cockles (33%), squid (38%) and prawn (44%) in supermarket. For wet market, V. cholerae only found in squid (18%) and no detection from prawn samples. For samples collected from Kuala Terengganu supermarket, the prevalence of V. cholera in cockle was at 29%, in squid at 56% and prawn at 75%. For samples collected from wet market, V. cholerae only present in squid at 18% and no detection in prawn samples. A total of 30 V. cholerae isolates were subjected to antibiotic testing. The isolates were completely resistance towards penicillin (100%), vancomycin (80%), tetracyline (23%), gentamycin (7%), norfloxacin (3%) and ciprofloxacin (3%). This study concluded prawn, cockle and squid samples from both district were contaminated with V. cholerae. Thus, it might pose risks to consumer if undercooked contaminated seafood were consumed.

Item Type: Article
Uncontrolled Keywords: Vibrio cholerae, seafood, Polymerase Chain Reaction (PCR), antibiotic resistance
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Bio-resources & Food Industry
Depositing User: Rafidah M.Saaid
Date Deposited: 14 Mar 2022 03:24
Last Modified: 14 Mar 2022 03:24

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