Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes

Nurul Hikmah, Harun and Abdi Wira, Septama and Ibrahim, Jantan (2015) Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes. Asian Pacific Journal of Tropical Biomedicine, 5 (1). pp. 48-53. ISSN 22211691

[img] Image
Restricted to Registered users only

Download (198kB) | Request a copy


Objective: To investigate the effects of 20 methanolic extracts from Malaysian selected plants on CD18/11a expression and phagocytosis activity of leukocytes using flow cytometry analysis. Methods: The effects of methanolic extracts on CD18/11a expression and phagocytosis of leukocytes were measured by labelling the cells with CD18-fluorescein isothiocyanate and ingestion labelled with Escherichia coli-fluorescein isothiocyanate and then analyzed using flow cytometer. Results: About 12 out of 20 methanolic extracts of selected Malaysian medicinal plants significantly (P≤0.05) inhibited the CD18/11a expression of leukocytes at both concentrations of 6.25 μg/mL and 100 μg/mL in dose dependent manner. The most active inhibitory was shown in Citrus aurantifolia (Christm.) Swingle and Alpinia galangal (L.) Willd. at dosage 100 μg/mL. Moreover, the Orthosiphon aristatus (Blume) Miq (O. aristatus). showed the highest stimulatory activity at the concentration of 100 μg/mL. Other than that, four plant extracts significantly (P≤0.05) rose the phagocytosis activities of leukocytes in dose dependent manner. However, Annona muricata L. and O. aristatus showed the highest stimulated activities at the 100 μg/mL concentration. Conclusions: The results suggest that methanolic extracts of Citrus aurantifolia, Alpinia galangal, O. aristatus and Annona muricata are able to modulate innate immune system and can potentially be recognized as therapeutic agents for modulating immune system.

Item Type: Article
Uncontrolled Keywords: Selected Malaysian medicinal plants, Immunomodulatory effects, CD18/11a expression, Phagocytosis activity, Flow cytometry analysis
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Health Sciences
Depositing User: Syahmi Manaf
Date Deposited: 13 Sep 2022 05:07
Last Modified: 13 Sep 2022 05:07

Actions (login required)

View Item View Item