Hexavalent molybdenum reduction to Mo-blue by Acinetobacter calcoaceticus

Suhaili, Zarizal and -, Shukor and -, M.Y.a and -, Rahman (2010) Hexavalent molybdenum reduction to Mo-blue by Acinetobacter calcoaceticus. Folia Microbiologica. pp. 137-143. ISSN 00155632

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A local molybdenum-reducing bacterium was isolated and tentatively identified as Acinetobacter calcoaceticus strain Dr.Y12 based on carbon utilization profiles using Biolog GN plates and 16S rDNA comparative analysis. Molybdate reduction was optimized under conditions of low dissolved oxygen (37 degrees C and pH 6.5). Of the electron donors tested, glucose, fructose, maltose and sucrose supported molybdate reduction after 1 d of incubation, glucose and fructose supporting the highest Mo-blue production. Optimum Mo-blue production was reached at 20 mmol/L molybdate and 5 mmol/L phosphate; increasing the phosphate concentrations inhibited the production. An increase in an overall absorption profiles, especially at peak maximum at 865 nm and the shoulder at 700 nm, was observed in direct correlation with the increased in Mo-blue amounts. Metal ions, such as chromium, cadmium, copper, mercury and lead (2 mmol/L final concentration) caused approximately 88, 53, 80, 100, and 20 % inhibition, respectively. Respiratory inhibitors, such as antimycin A, rotenone, sodium azide and cyanide showed in this bacterium no inhibition of the Mo-blue production, suggesting that the electron transport system is not a site of molybdate reduction.

Item Type: Article
Uncontrolled Keywords: EMTREE drug terms : molybdenum EMTREE medical terms:Acinetobacter calcoaceticus ;article;genetics;isolation and purification ; metabolism;molecular genetics;oxidation reduction reaction MeSH:Acinetobacter calcoaceticus ;Molecular Sequence Data;Molybdenum;Oxidation-Reduction Medline is the source for the MeSH terms of this document. SpeciesIndex :Acinetobacter calcoaceticus ;Bacteria (microorganisms)
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Bio-resources & Food Industry
Depositing User: Syahmi Manaf
Date Deposited: 13 Sep 2022 05:04
Last Modified: 13 Sep 2022 05:04
URI: http://eprints.unisza.edu.my/id/eprint/2920

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