Isolation and cloning of an inulinase gene from an endophytic bacteria

Ali, Abd Manaf and -, Basran and -, N.F.a and -, Mustafa (2010) Isolation and cloning of an inulinase gene from an endophytic bacteria. Advances in Environmental Biology, 4 (3). pp. 392-396. ISSN 19950756

[img] Image
Restricted to Registered users only

Download (214kB)
[img] Image
Restricted to Registered users only

Download (187kB)


In this study, an endophytic bacterium was isolated from Black Solenosternon scutellarioides plant and was named as AS. A simple and rapid method is described for the detection of inulinase-producing bacterial endophyte on agar plate using Remazol Brilliant Blue-inufin as substrate. The inulinase activity of AS bacteria isolate was detected by clear zone surroundings the colonies. The inulinase gene obtained from AS bacterium was amplified by the polymerase chain reaction. Degenerate primers were used specifically designed to pick up the inulinase gene. pGEM-T Easy and E. coli JM109 were used as cloning vector and host strain. respectively. The results showed that the partial sequence of inulinase gene consisted of 449bp. From the comparison with partial amino acid sequence of inulinase. the deduced polypeptide showed high sequence similarity to Paenibaccillus polymyxa and Geobacillus stearothermophilus exo-inulinases.

Item Type: Article
Uncontrolled Keywords: Endophytic bacteria; Gene cloning; lnulinase ; Oligosaccharides; RBB-inulin SpeciesIndex : Bacteria (microorganisms); Escherichia coli; Geobacillus stearothermophilus; Polymyxa; Solenostemon scutellarioides
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Bio-resources & Food Industry
Depositing User: Syahmi Manaf
Date Deposited: 13 Sep 2022 05:10
Last Modified: 13 Sep 2022 05:10

Actions (login required)

View Item View Item