Hexavalent molybdenum reduction to Mo-blue by Acinetobacter calcoaceticus

Zarizal, Suhaili (2010) Hexavalent molybdenum reduction to Mo-blue by Acinetobacter calcoaceticus. Folia Microbiologica. pp. 137-143. ISSN 00155632

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A local molybdenum-reducing bacterium was isolated and tentatively identified as Acinetobacter calcoaceticus strain Dr. Y12 based on carbon utilization profiles using Biolog GN plates and 16S rDNA comparative analysis. Molybdate reduction was optimized under conditions of low dissolved oxygen (37 °C and pH 6.5). Of the electron donors tested, glucose, fructose, maltose and sucrose supported molybdate reduction after 1 d of incubation, glucose and fructose supporting the highest Mo-blue production. Optimum Mo-blue production was reached at 20 mmol/L molybdate and 5 mmol/L phosphate; increasing the phosphate concentrations inhibited the production. An increase in an overall absorption profiles, especially at peak maximum at 865 nm and the shoulder at 700 nm, was observed in direct correlation with the increased in Mo-blue amounts. Metal ions, such as chromium, cadmium, copper, mercury and lead (2 mmol/L final concentration) caused .48, 53, 80, 100, and 20 % inhibition, respectively. Respiratory inhibitors, such as antimycin A, rotenone, sodium azide and cyanide showed in this bacterium no inhibition of the Mo-blue production, suggesting that the electron transport system is not a site of molybdate reduction. © 2010 Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic.

Item Type: Article
Uncontrolled Keywords: ELI TREE drug terms: molybdenum EMTREE medical terms: Acinetobacter calcoaceticus. article. genetics. isolation and purification. metabolism molecular genetics: oxidation reduction reaction MeSH: Acinetobacter calcoaceticus. Molecular Sequence Oata. Molybdenum: Oxidation-Reduction Mechne Is the source for the MeSH terms of !Ins document. Species Index: Acinetobacter calcoaceticus. Bacteria (microorganisms)
Subjects: S Agriculture > S Agriculture (General)
Divisions: Faculty of Bio-resources & Food Industry
Depositing User: Mrs Norhidayah Razak
Date Deposited: 29 Nov 2020 03:15
Last Modified: 15 Aug 2021 01:56
URI: http://eprints.unisza.edu.my/id/eprint/2011

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