A study of microbe air levels in selected rooms of a hospital cultivated on two culture medias [Kajian tahap mikrob dalam udara yang di kultur pada dua media kultur di dalam bilik hospital terpilih]

Hafizan, Juahir and Zarizal, Suhaili and Azman, Azid (2016) A study of microbe air levels in selected rooms of a hospital cultivated on two culture medias [Kajian tahap mikrob dalam udara yang di kultur pada dua media kultur di dalam bilik hospital terpilih]. Malaysian Journal of Analytical Sciences, 20 (5). pp. 1217-1224. ISSN 1394 - 2506

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Abstract

The levels of airborne microbe in hospital are unknown previously in Terengganu. Typically, fungi and bacteria are usually presented in indoor environments and cause of human health effects. The aim of this descriptive study was to investigate the level of airborne microbial pollution in the indoor air of the selected hospital rooms. A total of 8 rooms were investigated for this study. Sampling was conducted with an Eco-Mas 100 Single-Stage Microbial Air Impactor and counting plates containing two selective media, Rose Bengal Chloramphenicol Agar (RBCA) and Sabouraud Dextrose Agar (SDA). Air sampling was taken for 5 min at an airflow rate of 28.3 L/min. A medium-low level of bacterial and fungal concentrations (8 to 38 CFU/m3 for RBCA and 2 to 149 CFU/m3 for SDA), respectively were found in indoor air quality of the hospital. The highest microbe air levels for RBCA was measured in the washing room, while the highest microbe air levels for SDA was measured in the reprocessing room of hemodialysis unit. The culturable airborne bacterial and fungal concentrations on SDA agar were higher than those on RBCA. The most common culturable airborne microbes were Penicillium and Cladosporium. In addition, the use of RBCA rather than SDA significantly improved the fungal yield. The study also revealed that no indoor atmosphere in the hospital is completely free from microorganisms.

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