An Alternative Method of Induction of Type II Diabetes Mellitus in Sprague Dawley Rat

Holifa Saheera, Asmara and Ahmad Zubaidi, A.latif and Nasir, Mohamad (2020) An Alternative Method of Induction of Type II Diabetes Mellitus in Sprague Dawley Rat. Intern Med J, 25 (1). pp. 271-278. ISSN IMJ-06-01-2020-124

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Abstract

The induction of the type II diabetes model in Sprague Dawley rat is commonly performed for further laboratory experimental purposes. The main characteristic of type II diabetes mellitus is insulin resistance, which highly associated with high-fat and high-sugar diets. Therefore, to develop this condition, the current study proposed an alternative procedure of induction, wherein includes a combination of a highfat diet with multiple injections of streptozotocin and immediately, 5% glucose drinks were administered after the injection to enhance the hyperglycemic and insulin resistance state in the rat. This protocol successfully developed the hyperglycemic state. Poor glucose clearance indicated insulin resistance in the rat. In conclusion, this protocol provides an alternative procedure in inducing insulin resistance and hyperglycemic state of type II diabetes mellitus in Sprague Dawley rat.

Item Type: Article
Uncontrolled Keywords: Type II Diabetes Mellitus, Diabetic Induction, High-fat Diet, Streptozotocin
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Health Sciences
Depositing User: Fatin Safura
Date Deposited: 10 Apr 2022 02:02
Last Modified: 10 Apr 2022 02:02
URI: http://eprints.unisza.edu.my/id/eprint/6872

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