Mode of action of endophytic streptomyces sp., SUK 25 extracts against MRSA; microscopic, Biochemical and time-kill analysis

Hing Hiang, Lian and Dayang Fredalina, Basri and Noraziah, Mohamad Zin (2015) Mode of action of endophytic streptomyces sp., SUK 25 extracts against MRSA; microscopic, Biochemical and time-kill analysis. International Journal of Pharmaceutical Sciences Review and Research, 30 (1). pp. 11-17. ISSN 0976044X [P]

Image FH02-FPSK-15-02549.jpg Restricted to Registered users only Download (192kB) | Request a copy

Abstract

Bioactive compound from endophytic Streptomyces sp. has been claimed as a source of antibiotic. This study focused on the investigation of pharmacodynamic pattern and visualization of the mechanism of SUK 25 extracts against MRSA. The pharmacodynamic characteristic of the extract against MRSA 43300 was determined using time-kill assay. Then, the mode of action of the extracts was observed through biochemical assay and transmission electron microscopy. The SUK 25 extracts displayed bacteriostatic mode of action and concentration-dependent manner. The action of SUK 25 extracts against MRSA ATCC 43300 caused irregular shape of cells, which affected changes in Crystal Violet uptake by the cells. The release of UV absorbing materials and protein from the cells was caused by cell lysis. In conclusion, the action of the SUK 25 extracts against MRSA ATCC 43300 led to the internal change in cells, through which permeability of cells was altered by a decrease in the Crystal Violet uptake, shape of cell changes, and in turn brought about the lysis of cell.

Actions (login required)

View Item