Effect of non-hydrogen peroxide on antibacterial activities of Meliponini and Apis Honeys against Staphylococcus Aureus

Mohd Hilmi, Abu Bakar and Fatima, Ibrahim Jibril and Lavaniya, Manivannan (2017) Effect of non-hydrogen peroxide on antibacterial activities of Meliponini and Apis Honeys against Staphylococcus Aureus. Research Journal of Pharmacy and Technology, 10 (12). p. 8. ISSN 0974-3618

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Abstract

Honey is one of the greatest natural sources, enables to eliminate decay from infectious microorganisms due to effect of non-hydrogen peroxide. The study aims to explore the effect of non-hydrogen peroxide derived from Meliponini and Apis honeys against Staphylococcus aureus. Meliponini honey from stingless bee and Apis honey from non-stingless bee were used in the analysis of agar well diffusion assay that contained Staphylococcus aureus grown cultures. An amplex red hydrogen peroxide kit was used to identify the presence of hydrogen peroxide in the honey. Meanwhile, non-hydrogen peroxide activity was identified after catalase treatment. Hydrogen peroxide was absent in all Meliponini honeys however, it was present in Apis honey. Antibacterial activity of Meliponini honey is solely affected by non-hydrogen peroxide. Meanwhile, diverse effect between hydrogen peroxide and non-hydrogen peroxide on antibacterial activity in Apis honey.

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