Identification and quantification of Saponin in Furcraea gigantae

Norhayati, Ngah and Salmah, Mohamed and Syahril Amin, Hashim and Mohd Rohaizad, Md. Rejab (2021) Identification and quantification of Saponin in Furcraea gigantae. In: ICAFT 2021, 02-03 Mac 2021, UniSZA.

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Abstract

Furcraea plants contains saponins compound that can act as molluscicide agents and effective in controlling Golden Apple Snail at paddy field. However, the type of saponin and their amount is differed between species. This study was done to identify the type of saponin in Furcraea gigantae by using Fourier Transform Infrared (FTIR) spectroscopy. The amount of saponin in F. gigantae crude extract was quantify by using High Performance Liquid Chromatography (HPLC). Result shows that the F. gigantae has bidesmosidic furostanol saponin. The structure of furostanol saponin in F. gigantae was established as 3-[(O-6-deoxyα-L-mannopyranosyl-(1→4)-O-β-D-glucopyranos-yl-(1→3)-O-[O-β-D-glucopyranosyl-(1→3)- β-D-glucopyranosyl-(1→2)-O-β-D-glucopyranosyl-(1→4)-β-D-galactopyranosyl)oxy]-(3 β,5α,15α,22α,25R)-26-(β-D-glucopyranosyloxy)-15,22-di-hydroxy-furost-12-one (Da Silva and Parente, 2006). The yield of saponin extracted from the F. gigantae plant is 5.77 wt%. Findings is important in formulating the biopesticide made from F. gigantae.

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