Isolation and characterization of effective microorganism from fermented fruit juice

Norzilawati, Pauzi and Norhayati, Ngah and Abd. Jamil, Zakaria (2021) Isolation and characterization of effective microorganism from fermented fruit juice. In: ICAFT 2021, 02-03 Mac 2021, UniSZA.

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Abstract

Current study was conducted to isolate and characterized the bacteria from fermented fruit juice of papaya and pineapple using morphological and biochemical test. Isolated bacteria were cultured on selective medium i.e Burk medium, Pikovskaya agar, Aleksandrow agar, , gelatin supplemented agar and tryptic soy broth medium with L-tryptophan to evaluate their ability to fix nitrogen, solubilized phosphate, potassium and gelatin, as well as produced indole-3-acetic acid respectively. A total of 6 bacterial strains were isolated from papaya (3) and pineapple (3) respectively. All isolates were found to have the ability to fix nitrogen and produced IAA, five out of six bacteria capable of solubilized phosphate, while only one out of six isolates were able to solubilize potassium. Among isolates, bacteria from papaya named SB1 showed an outstanding characteristic comparable to control. This study showed that the isolated bacteria had potential to be exploited as an alternative biofertilizer and is worth for further investigation.

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