Protease-activated receptor 2 expression in the mammary gland tissues in correlation with mastitis severity in goats

Mohd Faizal, Ghazali and Siti Mariam, Zainal Ariffin (2021) Protease-activated receptor 2 expression in the mammary gland tissues in correlation with mastitis severity in goats. In: 2nd International Conference on Animal Production for Food Sustainability, 16 Jun 2021, Bali, Indonesia.

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Abstract

Mastitis is a common disease in small ruminant industry. The present study aimed to determine the presence of protease activated receptor-2 (PAR2) mRNA expression in the mammary gland of mastitis challenged goats. 30 clinically healthy mix breed lactating goats were divided into three groups, consisting of Staphylococcus aureus (Group 1), methicillin-resistant S. aureus (Group 2) and sterile phosphate-buffered saline (Control) groups. The data regarding physical condition of udder and clinical parameters of goats were recorded while milk samples and mammary gland tissues were collected at 24 and 48 hours post infection. Somatic cell count (SCC) was measured by direct microscopic method. The presence of PAR2 mRNA in the mammary gland tissue samples was detected by real-time PCR. Goats from group 1 developed mild to moderate clinical signs while Group 2 exhibited moderate to severe clinical signs. SCC was higher in both challenged groups than control group. PAR2 mRNA expression was detected in all mammary gland samples from Group 1 and Group 2. The gene expression was significantly highly in mammary gland tissue with severe clinical signs. The finding of PAR2 expression in caprine mammary gland is novel and important, suggesting serine proteases involved the development of mastitis in goat.

Item Type: Conference or Workshop Item (Paper)
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SF Animal culture
Divisions: Faculty of Bio-resources & Food Industry
Depositing User: Fatin Safura
Date Deposited: 04 Jan 2022 02:44
Last Modified: 04 Jan 2022 02:44
URI: http://eprints.unisza.edu.my/id/eprint/4350

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