Phytochemical screening, total flavonoid and phenolic content assays of various solvent extracts of musa sapientum

Thant, Zin and Mahadeva Rao, U.S. and Sundaram, M. Bala (2019) Phytochemical screening, total flavonoid and phenolic content assays of various solvent extracts of musa sapientum. In: International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS), 14-15 Sep 2019, Bedong, Kedah.

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Abstract

Flavonoids represent the most common and widely distributed group of plant phenolics and are abundant in foods; quercetin and rutin are the flavonoids most abundantly consumed. Musa sapientum is one of the well-known plants of the Musaceae family that have been used in traditional medicine since hundred years to alleviate various diseases and health problems. The objective of this research is to conduct the preliminary phytochemical screening, total flavonoid and phenolic contents assays of various solvent extracts of tepal of Musa sapientum. Phytochemical screening was carried out according to the method of Trease and Evans, total flavonoid content was measured by the aluminium chloride colorimetric assay and total phenolic content was estimated spectrophotometrically by FolinCiocalteau method. Preliminary phytochemical screening reveals the presence of phenolics, flavonoids, alkaloids, tannins, terpenoids in all the three different extracts (methanolic, ethanolic and aqueous). Polar solvents are frequently used for recovering polyphenols from plant matrices. The most suitable solvents are aqueous mixtures containing ethanol, methanol, acetone, and ethyl acetate. Tepal methanolic extract has the richest content of both phenolics and flavonoids i.e. (5.17 mg GAE/g and 0.36 mg QE/g) respectively, and aqueous extract was the least i.e. (2.02 mg GAE/g and 0.214 mg QE/g). All the extracts were not significantly different with one another (p>0.05). It can be hypothesised that the high contents of phenolic compounds of tepals of Musa sapientum indicated that these compounds contribute to the antioxidant activity and can be regarded as promising plant species for natural sources of radical scavenging activity with potential value for treatment of many life threatening diseases. The process of extraction and identification of active principles responsible for the free radical scavenging property of tepal extract of Musa sapientum through bioactivity guided fraction is under progress in order to understand the possible mechanism of action. Utilization of this tepal will be of advantage to mankind and increased in its consumption will help in prevention of chronic life style diseases.

Item Type: Conference or Workshop Item (Paper)
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Medicine
Depositing User: Muhammad Akmal Azhar
Date Deposited: 27 Jan 2021 03:53
Last Modified: 27 Jan 2021 03:53
URI: http://eprints.unisza.edu.my/id/eprint/2535

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