Betulinic acid inhibits growth of cultured vascular smooth muscle cells in vitro by inducing G1 arrest and apoptosis

Abd Manaf, Ali and Raja Kumar, Vadivelu and Swee, Keong Yeap and Muhajir, Hamid (2012) Betulinic acid inhibits growth of cultured vascular smooth muscle cells in vitro by inducing G1 arrest and apoptosis. Evidence-based complementary and alternative medicine. ISSN 1741427X

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Abstract

Betulinic acid inhibits growth of cultured vascular smooth muscle cells in vitro by inducing G1 arrest and apoptosis (Article) Vadivelu, R.K.a u, Yeap, S.K.a g2, Ali, A.M.b erd, Hamid, M.c u, Alitheen, N.B.d it a Institute of Bioscience, Universiti Putra Malaysia, Selangor, Serdang, Malaysia b Faculty of Agriculture and Biotechnology, Universiti Sultan Zainal Abidin, Kampus Kota, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Malaysia Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, 43400 Serdang, Malaysia View additional affiliations Abstract V View references (30) Betulinic acid is a widely available plant-derived triterpene which is reported to possess selective cytotoxic activity against cancer cells of neuroectodermal origin and leukemia. However, the potential of betulinic acid as an antiproliferative and cytotoxic agent on vascular smooth muscle (VSMC) is still unclear. This study was carried out to demonstrate the antiproliferative and cytotoxic effect of betulinic acid on VSMCs using 3-[4,5-dimethylthizol-2- y11-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry cell cycle assay, BrdU proliferation assay, acridine orange/propidium iodide staining, and comet assay. Result from MTT and BrdU assays indicated that betulinic acid was able to inhibit the growth and proliferation of VSMCs in a dose-dependent manner with IC50 of 18 g/mL significantly (13<0.05). Nevertheless, betulinic acid exhibited G1 cell cycle arrest in flow cytometry cell cycle profiling and low level of DNA damage against VSMC in acridine orange/propidium iodide and comet assay after 24 h of treatment. In conclusion, betulinic acid induced Gi cell cycle arrest and dose-dependent DNA damage on VSMC. 2012 Raja Kumar Vadivelu et al. Reaxys Database Information

Item Type:	Article
Uncontrolled Keywords:	EMTREE drug terms: acridine orange; betulic acid; broxuridine; propidium iodide; rapamycin EMTREE medical terms: antiproliferative activity; apoptosis; article; cell assay; cell culture; cell growth; comet assay; controlled study; cytotoxicity; drug inhibition; flow cytometry; G1 phase cell cycle checkpoint; growth inhibition; human; human cell; human tissue; IC 50; in vitro study; priority journal; smooth muscle fiber; vascular smooth Cited by 3 documents since 1996 Betulinic acid in complex with a gamma-cyclodextrin derivative decreases proliferation and in vivo tumor development of non-metastatic and metastatic B164A5 cells Soica, C. , Danciu, C. , Savoiu-Bant, G. (2014) International Journal of Molecular Sciences A Review on a Mangrove Species from the Sunderbans, Bangladesh: Barringtonia racemosa (L.) Roxb Zahirul Kabir, II. Mizanur Rahman, S. , Rashedul Islam, Id. (2013) American-Eurasian Journal of Sustainable Agriculture Polarized neural stem cells derived from adult bone marrow stromal cells develop a rosette-like structure Darabi, S. , Tiraihi, T. , Ruintan, A. (2013) In Vitro Cellular and Developmental Biology -Animal View all 3 citing documents Inform me when this document is cited in Scopus: ilpir Set citation alert I 0 Set citation feed Related documents A review on extraction, synthesis and anticancer activity of betulinic acid Tripathi, L. , Kumar, P. , Singh, R. (2009) Current Bioactive Compounds Betulinic acid, a natural compound with potent anticancer effects Mullauer, F.B. , Kessler, J.H. , Medema, J.P. (2010) Anti-Cancer Drugs Betulinic acid: A natural product with anticancer activity Fulda, S. (2009) Molecular Nutrition and Food Research
Subjects:	R Medicine > R Medicine (General)
Divisions:	Faculty of Bio-resources & Food Industry
Depositing User:	Mrs Norhidayah Razak
Date Deposited:	12 Jan 2021 02:31
Last Modified:	21 Aug 2021 08:21
URI:	http://eprints.unisza.edu.my/id/eprint/2444

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