Comparison of methicillin-resistant and methicillin-sensitive Staphylococcus aureus strains isolated from a tertiary hospital in Terengganu, Malaysia

Yeo, Chew Chieng (2012) Comparison of methicillin-resistant and methicillin-sensitive Staphylococcus aureus strains isolated from a tertiary hospital in Terengganu, Malaysia. Japanese journal of infectious diseases. pp. 502-509. ISSN 13446304

	Text FH02-FPBSM-14-00628.pdf Restricted to Registered users only Download (325kB)
	Image FH02-FPBSM-14-01140.jpg Restricted to Registered users only Download (267kB)

Abstract

SUMMARY Staphylococcus aureus is a persistent human pathogen responsible for a variety of infections ranging from soft-tissue infections to bacteremia The objective of this study was to determine genetic relatedness between methicillin-resistant S aureus (MRSA) and methicillin-susceptible S aureus (MSSA) strains We isolated 35 MRSA and 21 MSSA strains from sporadic cases at the main tertiary hospital in Terengganu Malaysia screening them for the presence of virulence genes Their genetic relatedness was determined by accessory gene regulator (agr) types. PCR-restriction fragment length polymorphism (RFLP) of the coa gene. pulsed-field gel electrophoresis (PFGE). S aureus protein A (spa). and multilocus-sequence typing (MLST) We found that 57% of MRSA and 43% of MSSA strains harbored enterotoxin genes. The majority (87.5%) of the strains were agr type I PCR-RFLP and PFGE genotyping of the coa gene revealed that MRSA strains were genetically related. whereas MSSA strains had higher heterogeneity The combined genotype MLST-spa type ST239-t037 was shared among MRSA and MSSA strains indicating that MRSA strains could have evolved from MSSA strains Two combined MLST-spa types were present in MRSA strains whereas 7 different MLST-spa types were detected in MSSA strains including 2 combined types (ST7794878 and ST1179-t267) that have not been reported in Malaysia In conclusion. enterotoxin genes were more prevalent in MRSA than in MSSA strains in the Terengganu hosptal. The MSSA strains were genetically more diverse than the MRSA strains

Item Type:	Article
Uncontrolled Keywords:	Staphylococcus enterotoxin. staphylococcus protein A EMTREE medical terms: article bactenal gene bacterial strain. bactenal virulence- bacterium identification; bacterium isolation. coa gene- enterotoxin gene genetic heterogeneity. genetic screening. genetic variability. Malaysia. methicillin resistant Staphylococcus aureus. methicillin susceptible Staphylococcus aureus molecular phylogeny. multilocus sequence typing. nonhuman. polymerase chain reaction. pulsed field gel electrophoresis. restriction fragment length polymorphism. tertiary health care
Subjects:	T Technology > T Technology (General)
Divisions:	Faculty of Bio-resources & Food Industry
Depositing User:	Mrs Norhidayah Razak
Date Deposited:	11 Jan 2021 03:49
Last Modified:	21 Aug 2021 08:44
URI:	http://eprints.unisza.edu.my/id/eprint/2441

Actions (login required)

View Item