Immunomodulatory effect of Rhaphidophora korthalsii on mice splenocyte, thymocyte and bone marrow cell proliferation and cytokine expression

Abd Manaf, Ali (2011) Immunomodulatory effect of Rhaphidophora korthalsii on mice splenocyte, thymocyte and bone marrow cell proliferation and cytokine expression. African journal of biotechnology. pp. 10744-10751. ISSN 16845315

Image FH02-FPBSM-14-00427.png Restricted to Registered users only Download (227kB)

Abstract

Rhaphidophora korthalsii methanol extract was previously found as a potential in vitro immunomodulating agent. In this study, the in vitro immunomodulatory activity of R. korthalsii methanol extract on mice splenocyte were quantified through evaluating T/NK cell population. cytokine expression (IL-2 and IFN-y) and cytotoxicity against Yac-1 cell In vitro MTT proliferation study showed that R. korthalsii methanol extract stimulate significant proliferation of splenocyte. thymocyte and bone marrow cell at 25 pg/ml after 72 h incubation as compared to rIL-2. Besides. 25 lig/ma of extract also enhanced T-helper 1 cytokine (IL-2 and IFN-y) secretion. natural killer cell (NK1.1 *CO3-) population and cytotoxicity against natural killer sensitive Yac-1 leukemic cell line. It was concluded that R. korthalsii methanol extract is a potential immunomodulator agent that could activate the natural killer cell. 0 2011 Academic Journals_ Author keywords

Actions (login required)

View Item