A specific non-coding RNA in extracellular vesicles from induced neuronal cells and epigenetic regulation of neurotransmission

Norhidayah, Badya and Tedford, E. and Laing, C. and McConkey, G. (2020) A specific non-coding RNA in extracellular vesicles from induced neuronal cells and epigenetic regulation of neurotransmission. In: International Society for Extracellular Vesicles ISEV2020 Annual Meeting, 20-22 Jul 2020.

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Abstract

Introduction: Our late-breaking finding is the identi- fication of a non-coding RNA (ncRNA) in extracellular vesicles (EVs) from neuronal cells that is a natural antisense transcript for the DBH gene and associated with epigenetic changes and gene silencing. DNA methylation in neurons is involved in memory and neurological disorders (Science 2018 361 (6409)). Earlier work found that during chronic brain infection with Toxoplasma gondii induced a decrease in norepinephrine levels and expression of the host DBH gene; and the decrease is correlated with behaviours linked to noradrenergic signalling (Infect Immun. 2019 87(2); Infect Immun. 2016 84(2861)). DBH catalyzes the pro- duction of norepinephrine from dopamine in noradre- nergic neurons. We found that EVs from infected cultures suppress transcription of the DBH gene and hypermethylation of the gene in noradrenergic cells in vitro. In this study, we identify a ncRNA in the EVs from infected neuronal cells.

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