Detection and characterization of vibrio parahaemolyticus in raw mackerel fish and fish-based street food

Syamimi Hanim, M. and Tan, J.Y. and Ho, L.H. and Kamarul'Ain, M. (2018) Detection and characterization of vibrio parahaemolyticus in raw mackerel fish and fish-based street food. In: International Conference on Agriculture, Animal Sciences and Food Technology (ICAFT) 2018, 17 Dec 2018, UNISZA.

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Abstract

This study aimed to investigate the prevalence of Vibrio parahaemolyticus in raw mackerel fish as well as fish-based products namely satar and otak-otak. A total of 120 fish, 90 satar and 35 otak-otak that have been purchased randomly in Terengganu were subjected to PCR and conventional. plating method for detection and isolation of V parahaemo!J1ticus. The isolates were characterized by 12 types of antibiotic using discs diffusion method. About 67.5%, 23.3% and 5.71% of fish, satar and otak-otak were contaminated with V parahaemolyticus. A total of 58 V parahaemolyticus isolates were recovered from fish. Among them, 24 of V parahaemolyticus isolates carried tdh gene and 3 isolates had trh gene. All the isolates were susceptible to amikacin, norfloxacin, ceftazidime, ciprofloxacin, and enrofloxacin. There was high resistant towards penicillin (93.1%), vancomycin (89.7%) and ampicillin (55.2%). About 56.9% of strains isolated showed a MAR index higher than 0.2, indicating these strains were from high risk sources of contamination. This study found V parahae111o!Jticus contaminated raw fish mackerel, satar and otak-otak.

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