The Val66Met polymorphism in the brain derived neurotphic factor (BDNF) gene and it is potential association with stress levels and haematological parameters

Atif, Amin Baig and Imilia, Ismail and Tengku Mohammad Ariff, Raja Hussin and Mohammad Ahmed, Isa and Omar Alshajrawi, Mohd (2018) The Val66Met polymorphism in the brain derived neurotphic factor (BDNF) gene and it is potential association with stress levels and haematological parameters. In: 3rd International Symposium on Advances in Molecular Biology of Plants and Health Sciences, 19 Dec 2018, Lahore, Pakistan.

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Abstract

The BDNF gene (Val66Mct) polymorphism plays a significant role in genetic predisposition towards stress. The stressful lifestyle of preclinical medical students increases stress levels, which is hyphothesized to increase oxidative stress and in turn can lead to alteration in the haematological parameters. This study was aimed to determine allelic and genotypic association or the Val66Met polymorphism with level of stress and to assess the haematological parameters in preclinical medical students or Universiti Sultan Zainal Abidin, Malaysia. A cross-sectional study was conducted 122 preclinical medical students. The validated depression anxiety stress mucs-21 (DASS-21) questionnaire was distributed, and blood samples were collectcd to perform complete blood count (CBC) test and polymerase chain reaction-restriction fragment length polymorphism (PCR· RFLP). Results showed that BDNF genotypes differed significantly with perceived stress level (p=0.010); the students who carried Val/Val genotype have perceived significantly lower stress than those students who carried Val/Met and Mel/Mel genotypes, respectively. Those students with Val/Met genotype have significantly greater haemoglobin concentration than those with Mel/Met genotype (p=0.044). There was a statistically significant difference in the results of mean corpuscular haemoglobin concentration (MCHC) across their BDNF genotypes (p=-0.036). There was a statistically significant negative association with increasing stress to their white blood cells (WBCs) count (p=0.05). The data generated from this study will be complementing attention from the investigators to focus on the impact of stress and oxidative stress on haematological parameters under potential genes.

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